Composite

Part:BBa_K092700

Designed by: EPF-Lausanne Team 2008   Group: iGEM08_EPF-Lausanne   (2008-10-19)


TetR cds with RBS and Terminator + pTetR, RFP with RBS + LuxI with RBS and terminator

Combination of the following parts : BBa_K092000, BBa_K092300 and BBa_K092400: The TetR sequence (with a ribosome binding site and terminator)[BBa_K09200] is bound to the part containing the promoter of TetR and the red fluorescent protein (RFP) with a ribosome binding site. A luxI part (with RBS and terminator) is added at the end of the cds of the RFP. As no terminator was placed after the RFP, they both depends on the TetR promoter. The transcription of RFP and LuxI is inhibited when TetR is present. The production of TetR depends on the promoter you put in front of it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1483
    Illegal AgeI site found at 1595
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None